On 5.15 (9 days ago) we (Kiez Pilz) attempted to clone mycelium into agar from a wild oyster mushroom that Logan and Ann foraged on a hike, and from a yellow oyster mushroom which they grew.
Prior to cloning, the wild oyster had been stored in the fridge for one week.
At Top Lab, we poured agar into plastic and glass petri dishes (using a soy-molasses-agar recipe), and then transferred mycelium pieces into the plates in the flow hood, letting each mycelium piece sit in hydrogen peroxide for 5 minutes before putting it into the agar (will link to the protocols here).
After four days we saw some mycelial growth (and also some contaminations), in both yellow and wild oyster.
There was no contamination in the petri dishes we poured which we did not transfer into.
We also used a couple petri dishes which were professionally poured by the TU lab in Berlin and given to Top Lab. These dishes also contain anti-biotics and I do not know the exact recipe they used for the medium.
Due to life events and busyness, I was not able to check on the petri dishes again until today (9 days after the transfer) and there is no significant growth and contaminations. Here are the photos:
[1] Yellow-oyster-TU-pour:
[2] Yellow-oyster-KPK-pour:
[3] yellow-oyster-kpk-pour
[4] wild oyster tu-pour
[5] wild oyster kpk-pour
[6] wild oyster kpk pour
[7] wild oyster TU pour
going to try to transfer and isolate today!
would be curious to hear thoughts on contaminations,
also wondering if this super fluffy stuff is just mycelium… it seems a bit 3 dimensional