Missed some updates! Reconstructing below:
New culture started from spore syringe. Pleurotus Columbinus (Blue Oyster) transferred to 3 blank agar plates in SAB. Droplet transferred to flame-sterilized inoculation loop and spread in zig-zag pattern. Labelled C1.
Some Mysterious Day in Late May
Lentinula Edodes (Shiitake): Transfer of half of colonized wood dowels to old logs gathered last season. Do they have another fungus which has already visibly colonized? Yes. Are these the least visibly colonized of the logs. Yes. Do I dare to dream? Yes. Logs drilled and dowels hammered in. Plugs covered with candlewax. Set aside in the corner of garden storage space standing upright together. Let’s just see what happens in a year or whenever.
Pleurotus Columbinus: Two C1 plates remained blank–no growth whatsoever. Third C1 plate fully colonized (and then a few extra days of my own delay). No contamination. Transfer of agar wedges from two most vigorous growth zones from third C1 plate to two new blank plates, labelled C2 in SAB. Flame-sterilized scalpel used for transfer; not a new blade. C1 plate placed in cold storage.
Pleurotus Columbinus: C2 plates fully grown. No contamination. Transfer of scraped growth from most vigorous zone in each plate transferred to 1 new blank plate each in SAB, labeled C3. Flame-sterilized inoculation loop used for transfers. C2 plates placed in cold storage.
General Lab Procedure: Two jars of honey liquid culture mixed. 350 mL, with addition of ~.025 g gypsum, ~2 g sawdust, glass shard in each. Sterilized in pressure cooker for a little less than 1.5 hours above 15 psi. (Previous sterilization over 1.5 hours lead to some carmelization). Moxed supplemented sawdust, 10:2:1/8::sawdust:wheat bran:gypsum. Can’t remember specific amounts mixed dried, but hydrated to feel and separated into four jars. Sterilized for ~3 hours above 15 psi.
Pleurotus Columbinus: Both C3 plates nearly fully grown. 1 plate presenting possible sectoring. Uniform growth plate chosen for transfer to liquid culture. Relabeled P1. Agar wedge transfer in SAB from P1 to two jars of honey liquid culture via flame sterilized scalpel (not a new blade). P1 and remaining C3 placed in cold storage.
Lentinula Edodes (Shiitake): 2nd batch of wood dowels transferred to 2 recently cut logs. Logs are clear of fungi growth, but came from a dead tree killed by insect infestation. Drilled and hammered in; plugs covered with candlewax. No major investment in this project. Doused in remaining liquid culture because: ¯_ (ツ)_/¯