A couple weeks ago I made some liquid culture,
using 1000ml water and 8g honey as media ratio,
in jars with airport lids.
I inoculated the liquid media using slices of agar of oyster mycelium.
Everyday I put the jar on a magnetic stirrer until it whirlpools (for about 10-30 seconds),
and there is a metal bolt in the jar which helps it stir.
After about 2 weeks, here is what I am seeing:
I have a couple questions:
- what should the liquid culture look like when it is done / ready for use to inoculate other media? do these jars looks good?
- is this one whirlpool a day enough stirring?
I also tried inoculating some petri dishes with some of the liquid culture
– I maybe put too much LC into the dishes, but it appears to me that there is a layer of mycelium growing over the entire dish.